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OriGene human gfp cdna
Human Gfp Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+gfp+cdna/Female+Human+Forensic+Control+DNA/us12606846-239-1-8
Average 90 stars, based on 1 article reviews

human gfp cdna - by Bioz Stars, 2026-10

90/100 stars

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Article Title: Materials and methods for the treatment of disorders associated with the IRF2BPL gene
Article Snippet: The human GFP cDNA clone was obtained from Origene, Rockville, Md.

Image Search Results

Volcano plot showing LC–MS/MS–based proteomic analysis of GFP pull-down samples from HEK293T cells expressing GFP-tagged wild-type (WT) or mutant LRRC8B. The x-axis represents log₂ fold change (mutant vs WT), and the y-axis shows −log₁₀ adjusted p-value. Proteins enriched in WT samples are shown on the left, while those enriched in mutant samples are shown on the right. Selected significantly enriched proteins are labeled. VDAC1 is identified as a WT-enriched interactor.

Journal: bioRxiv

Article Title: A neuropsychiatric disease-associated mutation in LRRC8B disrupts cellular Ca²⁺ signaling, mitochondrial function, and bioenergetics

doi: 10.64898/2026.04.16.718892

Figure Lengend Snippet: Volcano plot showing LC–MS/MS–based proteomic analysis of GFP pull-down samples from HEK293T cells expressing GFP-tagged wild-type (WT) or mutant LRRC8B. The x-axis represents log₂ fold change (mutant vs WT), and the y-axis shows −log₁₀ adjusted p-value. Proteins enriched in WT samples are shown on the left, while those enriched in mutant samples are shown on the right. Selected significantly enriched proteins are labeled. VDAC1 is identified as a WT-enriched interactor.

Article Snippet: The plasmid encoding GFP-tagged human LRRC8B (HG24935-ACG; Sino Biological Inc, USA).

Techniques: Liquid Chromatography with Mass Spectroscopy, Expressing, Mutagenesis, Labeling

$Representative immunoblot images showing VDAC protein levels in total cell lysates and isolated mitochondrial fractions from cells transfected with GFP-tagged LRRC8B WT (W) or mutant Y380S (M) constructs. Actin (∼42 kDa) is included as a loading control for total lysates. Red boxes indicate the regions that were cropped and presented in the main figures. Molecular weight markers are shown where applicable.$

Journal: bioRxiv

Article Title: A neuropsychiatric disease-associated mutation in LRRC8B disrupts cellular Ca²⁺ signaling, mitochondrial function, and bioenergetics

doi: 10.64898/2026.04.16.718892

Figure Lengend Snippet: Representative immunoblot images showing VDAC protein levels in total cell lysates and isolated mitochondrial fractions from cells transfected with GFP-tagged LRRC8B WT (W) or mutant Y380S (M) constructs. Actin (∼42 kDa) is included as a loading control for total lysates. Red boxes indicate the regions that were cropped and presented in the main figures. Molecular weight markers are shown where applicable.

Article Snippet: The plasmid encoding GFP-tagged human LRRC8B (HG24935-ACG; Sino Biological Inc, USA).

Techniques: Western Blot, Isolation, Transfection, Mutagenesis, Construct, Control, Molecular Weight

(A) Representative immunoblot images showing protein levels of GFP-tagged LRRC8B (WT and mutant) and VDAC in input lysates used for pull-down assays. (B–C) Immunoblot analysis of four independent immunoprecipitation (IP) experiments. Bands at ∼120 kDa confirm successful pull-down of GFP-tagged LRRC8B (WT and mutant) using an anti-GFP antibody. VDAC (∼32 kDa) bands indicate co-precipitation of VDAC with LRRC8B WT and mutant proteins. VDAC levels were normalized to the corresponding LRRC8B (WT or mutant) levels for quantification. Red boxes highlight the regions that were cropped and presented in the main figures. Molecular weight markers are shown where applicable.

Journal: bioRxiv

Article Title: A neuropsychiatric disease-associated mutation in LRRC8B disrupts cellular Ca²⁺ signaling, mitochondrial function, and bioenergetics

doi: 10.64898/2026.04.16.718892

Figure Lengend Snippet: (A) Representative immunoblot images showing protein levels of GFP-tagged LRRC8B (WT and mutant) and VDAC in input lysates used for pull-down assays. (B–C) Immunoblot analysis of four independent immunoprecipitation (IP) experiments. Bands at ∼120 kDa confirm successful pull-down of GFP-tagged LRRC8B (WT and mutant) using an anti-GFP antibody. VDAC (∼32 kDa) bands indicate co-precipitation of VDAC with LRRC8B WT and mutant proteins. VDAC levels were normalized to the corresponding LRRC8B (WT or mutant) levels for quantification. Red boxes highlight the regions that were cropped and presented in the main figures. Molecular weight markers are shown where applicable.

Article Snippet: The plasmid encoding GFP-tagged human LRRC8B (HG24935-ACG; Sino Biological Inc, USA).

Techniques: Western Blot, Mutagenesis, Immunoprecipitation, Molecular Weight

a , Retinal whole mounts from 6-week-old CX3CR1 GFP/GFP and C57BL/6J mice were stained with anti-CD68 and anti-Iba-1 antibodies. Representative confocal images focused on the OPL are shown. The white arrowheads indicate CD68 + /GFP + or CD68 + /Iba-1 + microglia. b , c , Quantification of GFP + or Iba-1 + ( b ) and CD68 + /GFP + or CD68 + /Iba-1 + microglia ( c ) ( n = 4 mice per group). d Retinal sections from CX3CR1 GFP/GFP and C57BL/6J mice were stained with anti-GFAP and anti-Iba-1 antibodies. The yellow arrow indicates a resting microglial cell, and the white arrows indicate the dendritic migration of activated microglia into the ONL and INL. The yellow arrowhead indicates a resting astrocyte, and the white arrowheads indicate the extension of the dendritic processes of activated astrocytes into the IPL. e , Quantification of activated microglia in the ONL of retinas from CX3CR1 GFP/GFP and C57BL/6J mice ( n = 5–7 mice per group). f , qPCR analysis of proinflammatory molecules, including Tnfα , Il1b , Il6 , Cox2 and Nos2 , and anti-inflammatory cytokines, including Il10 and Il13 , in CX3CR1 GFP/GFP and C57BL/6J mouse retinas ( n = 6 mice per group). g , Retinal sections from CX3CR1 GFP/GFP and C57BL/6J mice were stained with anti-R/G opsin and anti-Iba-1 antibodies. Yellow arrowheads indicate cone photoreceptors. The boxed regions are highly magnified at the bottom, showing cone photoreceptors. Yellow arrows indicate resting microglia, and white arrowheads indicate the dendritic migration of activated microglia into the ONL. h , Quantification of cone photoreceptor cells in retinal sections ( n = 4–6 mice per group). i , j , Representative ERG images of 6-week-old CX3CR1 GFP/GFP and C57BL/6J mice at 3 cd·s/m 2 under scotopic conditions ( i ) and at 10 cd·s/m 2 under photopic conditions ( j ). k , l , Amplitudes of ERG recordings under both scotopic and photopic conditions in 6-week-old CX3CR1 GFP/GFP and C57BL/6J mice ( n = 11 mice per group). The data are presented as the mean ± s.e.m. and were analyzed via unpaired two-tailed Student’s t -tests (CX3CR1 GFP/GFP versus C57BL/6J, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). INL, inner nuclear layer. Scale bar, 20 µm.

Journal: Experimental & Molecular Medicine

Article Title: Microglial CX3CR1 deficiency regulates the selective vulnerability of cone photoreceptors via STAT3/CCL–ACKR1 signaling in the mouse retina

doi: 10.1038/s12276-025-01618-7

Figure Lengend Snippet: a , Retinal whole mounts from 6-week-old CX3CR1 GFP/GFP and C57BL/6J mice were stained with anti-CD68 and anti-Iba-1 antibodies. Representative confocal images focused on the OPL are shown. The white arrowheads indicate CD68 + /GFP + or CD68 + /Iba-1 + microglia. b , c , Quantification of GFP + or Iba-1 + ( b ) and CD68 + /GFP + or CD68 + /Iba-1 + microglia ( c ) ( n = 4 mice per group). d Retinal sections from CX3CR1 GFP/GFP and C57BL/6J mice were stained with anti-GFAP and anti-Iba-1 antibodies. The yellow arrow indicates a resting microglial cell, and the white arrows indicate the dendritic migration of activated microglia into the ONL and INL. The yellow arrowhead indicates a resting astrocyte, and the white arrowheads indicate the extension of the dendritic processes of activated astrocytes into the IPL. e , Quantification of activated microglia in the ONL of retinas from CX3CR1 GFP/GFP and C57BL/6J mice ( n = 5–7 mice per group). f , qPCR analysis of proinflammatory molecules, including Tnfα , Il1b , Il6 , Cox2 and Nos2 , and anti-inflammatory cytokines, including Il10 and Il13 , in CX3CR1 GFP/GFP and C57BL/6J mouse retinas ( n = 6 mice per group). g , Retinal sections from CX3CR1 GFP/GFP and C57BL/6J mice were stained with anti-R/G opsin and anti-Iba-1 antibodies. Yellow arrowheads indicate cone photoreceptors. The boxed regions are highly magnified at the bottom, showing cone photoreceptors. Yellow arrows indicate resting microglia, and white arrowheads indicate the dendritic migration of activated microglia into the ONL. h , Quantification of cone photoreceptor cells in retinal sections ( n = 4–6 mice per group). i , j , Representative ERG images of 6-week-old CX3CR1 GFP/GFP and C57BL/6J mice at 3 cd·s/m 2 under scotopic conditions ( i ) and at 10 cd·s/m 2 under photopic conditions ( j ). k , l , Amplitudes of ERG recordings under both scotopic and photopic conditions in 6-week-old CX3CR1 GFP/GFP and C57BL/6J mice ( n = 11 mice per group). The data are presented as the mean ± s.e.m. and were analyzed via unpaired two-tailed Student’s t -tests (CX3CR1 GFP/GFP versus C57BL/6J, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). INL, inner nuclear layer. Scale bar, 20 µm.

Article Snippet: For the detection of TNF-α, CCL2, CCL3, CCL4 and CXCL12 expressions in cell supernatants from MACS-sorted microglia or astrocytes in CX3CR1 GFP/GFP and C57BL/6J retinas at 6 weeks of age, TNF alpha Mouse Uncoated ELISA Kit (Invitrogen), MCP-1/CCL2 Mouse Uncoated ELISA Kit (Invitrogen), Mouse CCL3/MIP-1 alpha Quantikine ELISA Kit (R&D system), Mouse CCL4/MIP-1 beta DuoSet ELISA (R&D system) and SDF-1 alpha/CXCL12 Mouse ELISA Kit (Invitrogen) were applied.

Techniques: Staining, Migration, Two Tailed Test

a , Volcano plot showing DEPs in retinas from 6-week-old CX3CR1 GFP/GFP and C57BL/6J mice ( n = 5 mice per group). Red dots indicate upregulated proteins (FC >1.5), and green dots indicate downregulated proteins (FC <1/1.5), with a Q value <0.05. b – d , GO analysis of DEPs, shown separately for biological process ( b ), cellular component ( c ) and molecular function ( d ), between CX3CR1 GFP/GFP retinas and C57BL/6J retinas. e , KEGG pathway analysis of DEPs between CX3CR1 GFP/GFP retinas and C57BL/6J retinas. f , PPI analysis of DEPs. Pink dots indicate upregulated proteins, and blue dots represent downregulated proteins. g , KEGG network analysis of DEPs. The purple squares indicate the KEGG pathways, and a deeper color indicates greater significance. The red dots indicate upregulated DEPs, and the blue dots indicate downregulated DEPs. h , i , Western blotting analysis ( h ) and quantification of p-STAT3/STAT3 expression ( i ) in retinas from 6-week-old CX3CR1 GFP/GFP and C57BL/6J mice ( n = 8–9 mice per group). j , Colocalization of p-STAT3 and Iba-1 in microglia from CX3CR1 GFP/GFP and C57BL/6J retinas at 6 weeks of age. The white arrowheads indicate p-STAT3 + /Iba-1 + microglia. Scale bar, 50 µm. k , Quantification of the percentages of p-STAT3 + /Iba-1 + microglia in j . l , qPCR analysis of Stat3 and proinflammatory molecule expression in microglia from CX3CR1 GFP/GFP and C57BL/6J retinas at 6 weeks of age. The results shown represent three to four independent experiments. The data are presented as the mean ± s.e.m. and were analyzed via unpaired two-tailed Student’s t -test (** P < 0.01, *** P < 0.001, **** P < 0.0001).

Journal: Experimental & Molecular Medicine

Article Title: Microglial CX3CR1 deficiency regulates the selective vulnerability of cone photoreceptors via STAT3/CCL–ACKR1 signaling in the mouse retina

doi: 10.1038/s12276-025-01618-7

Figure Lengend Snippet: a , Volcano plot showing DEPs in retinas from 6-week-old CX3CR1 GFP/GFP and C57BL/6J mice ( n = 5 mice per group). Red dots indicate upregulated proteins (FC >1.5), and green dots indicate downregulated proteins (FC <1/1.5), with a Q value <0.05. b – d , GO analysis of DEPs, shown separately for biological process ( b ), cellular component ( c ) and molecular function ( d ), between CX3CR1 GFP/GFP retinas and C57BL/6J retinas. e , KEGG pathway analysis of DEPs between CX3CR1 GFP/GFP retinas and C57BL/6J retinas. f , PPI analysis of DEPs. Pink dots indicate upregulated proteins, and blue dots represent downregulated proteins. g , KEGG network analysis of DEPs. The purple squares indicate the KEGG pathways, and a deeper color indicates greater significance. The red dots indicate upregulated DEPs, and the blue dots indicate downregulated DEPs. h , i , Western blotting analysis ( h ) and quantification of p-STAT3/STAT3 expression ( i ) in retinas from 6-week-old CX3CR1 GFP/GFP and C57BL/6J mice ( n = 8–9 mice per group). j , Colocalization of p-STAT3 and Iba-1 in microglia from CX3CR1 GFP/GFP and C57BL/6J retinas at 6 weeks of age. The white arrowheads indicate p-STAT3 + /Iba-1 + microglia. Scale bar, 50 µm. k , Quantification of the percentages of p-STAT3 + /Iba-1 + microglia in j . l , qPCR analysis of Stat3 and proinflammatory molecule expression in microglia from CX3CR1 GFP/GFP and C57BL/6J retinas at 6 weeks of age. The results shown represent three to four independent experiments. The data are presented as the mean ± s.e.m. and were analyzed via unpaired two-tailed Student’s t -test (** P < 0.01, *** P < 0.001, **** P < 0.0001).

Techniques: Western Blot, Expressing, Two Tailed Test

a , qPCR analysis of Stat3 and proinflammatory molecule expression in retinas from 6-week-old CX3CR1 GFP/GFP mice treated with siSTAT3 or siCTR ( n = 5 mice per group). b , Retinal whole mounts from CX3CR1 GFP/GFP mice treated with siSTAT3 or siCTR were stained with an anti-CD68 antibody. The white arrowheads indicate CD68-positive microglia. c , Quantification of CD68 + /GFP + microglia ( n = 6–7 mice per group). d , Retinal sections from siSTAT3- or siCTR-treated CX3CR1 GFP/GFP mice were stained with an anti-GFAP antibody. The white arrows indicate the extension of the dendritic processes of activated astrocytes into the IPL, and the white arrowheads indicate the dendritic migration of activated microglia into the ONL. e , Quantification of microglial cell density in the ONL of retinas from CX3CR1 GFP/GFP mice treated with siSTAT3 or siCTR ( n = 5 mice per group). f , Quantification of the mean immunofluorescence intensity of GFAP in retinal sections ( n = 5 mice per group). g , Quantification of cone photoreceptors in retinas from siSTAT3- or siCTR-treated CX3CR1 GFP/GFP mice ( n = 5 mice per group). h , Retinal sections from siSTAT3- or siCTR-treated CX3CR1 GFP/GFP mice were stained with an anti-R/G opsin antibody. Yellow arrowheads indicate cone photoreceptors. The boxed regions are shown at higher magnification at the bottom. The white arrowheads show the dendritic migration of activated microglia into the ONL. i , Retinal sections from CX3CR1 GFP/GFP mice after treatment with siSTAT3 or siCTR were stained with anti-Caspase-3 and anti-PNA antibodies. The white arrowheads indicate Caspase-3 + /PNA + cells. j , k , Quantification of the number of PNA + cells ( j ) and percentages of Caspase-3 + /PNA + cells ( k ) in retinal sections ( n = 5 mice per group). l , Quantification of the mean immunofluorescence intensity of GFAP ( n = 5 mice per group). m , Retinal sections from CX3CR1 GFP/GFP mice treated with AAV-F4/80p-siSTAT3-mCherry or AAV-F4/80p-siCTR-mCherry were stained with an anti-GFAP antibody. The white arrowheads show the migration of activated microglia in the ONL, and the white arrows indicate the dendritic extension of activated astrocytes into the IPL from the NFL. n , Retinal sections from 6-week-old CX3CR1 GFP/GFP mice treated with AAV-F4/80p-siSTAT3-mCherry or AAV-F4/80p-siCTR-mCherry were stained with an anti-R/G opsin antibody. The white arrowheads indicate the migration of activated microglia in the ONL, and the yellow arrowheads indicate the R/G opsin + cone photoreceptors. o , Quantification of the number of R/G opsin + cone photoreceptors in n ( n = 5 mice per group). p , ERG recordings of CX3CR1 GFP/GFP mice at 6 weeks of age treated with AAV-F4/80p-siSTAT3-mCherry or AAV-F4/80p-siCTR-mCherry ( n = 10 mice per group). Scale bar, 20 µm. The data are presented as the mean ± s.e.m. and were analyzed via unpaired two-tailed Student’s t -tests (* P < 0.05, ** P < 0.01, *** P < 0.001).

Journal: Experimental & Molecular Medicine

Article Title: Microglial CX3CR1 deficiency regulates the selective vulnerability of cone photoreceptors via STAT3/CCL–ACKR1 signaling in the mouse retina

doi: 10.1038/s12276-025-01618-7

Figure Lengend Snippet: a , qPCR analysis of Stat3 and proinflammatory molecule expression in retinas from 6-week-old CX3CR1 GFP/GFP mice treated with siSTAT3 or siCTR ( n = 5 mice per group). b , Retinal whole mounts from CX3CR1 GFP/GFP mice treated with siSTAT3 or siCTR were stained with an anti-CD68 antibody. The white arrowheads indicate CD68-positive microglia. c , Quantification of CD68 + /GFP + microglia ( n = 6–7 mice per group). d , Retinal sections from siSTAT3- or siCTR-treated CX3CR1 GFP/GFP mice were stained with an anti-GFAP antibody. The white arrows indicate the extension of the dendritic processes of activated astrocytes into the IPL, and the white arrowheads indicate the dendritic migration of activated microglia into the ONL. e , Quantification of microglial cell density in the ONL of retinas from CX3CR1 GFP/GFP mice treated with siSTAT3 or siCTR ( n = 5 mice per group). f , Quantification of the mean immunofluorescence intensity of GFAP in retinal sections ( n = 5 mice per group). g , Quantification of cone photoreceptors in retinas from siSTAT3- or siCTR-treated CX3CR1 GFP/GFP mice ( n = 5 mice per group). h , Retinal sections from siSTAT3- or siCTR-treated CX3CR1 GFP/GFP mice were stained with an anti-R/G opsin antibody. Yellow arrowheads indicate cone photoreceptors. The boxed regions are shown at higher magnification at the bottom. The white arrowheads show the dendritic migration of activated microglia into the ONL. i , Retinal sections from CX3CR1 GFP/GFP mice after treatment with siSTAT3 or siCTR were stained with anti-Caspase-3 and anti-PNA antibodies. The white arrowheads indicate Caspase-3 + /PNA + cells. j , k , Quantification of the number of PNA + cells ( j ) and percentages of Caspase-3 + /PNA + cells ( k ) in retinal sections ( n = 5 mice per group). l , Quantification of the mean immunofluorescence intensity of GFAP ( n = 5 mice per group). m , Retinal sections from CX3CR1 GFP/GFP mice treated with AAV-F4/80p-siSTAT3-mCherry or AAV-F4/80p-siCTR-mCherry were stained with an anti-GFAP antibody. The white arrowheads show the migration of activated microglia in the ONL, and the white arrows indicate the dendritic extension of activated astrocytes into the IPL from the NFL. n , Retinal sections from 6-week-old CX3CR1 GFP/GFP mice treated with AAV-F4/80p-siSTAT3-mCherry or AAV-F4/80p-siCTR-mCherry were stained with an anti-R/G opsin antibody. The white arrowheads indicate the migration of activated microglia in the ONL, and the yellow arrowheads indicate the R/G opsin + cone photoreceptors. o , Quantification of the number of R/G opsin + cone photoreceptors in n ( n = 5 mice per group). p , ERG recordings of CX3CR1 GFP/GFP mice at 6 weeks of age treated with AAV-F4/80p-siSTAT3-mCherry or AAV-F4/80p-siCTR-mCherry ( n = 10 mice per group). Scale bar, 20 µm. The data are presented as the mean ± s.e.m. and were analyzed via unpaired two-tailed Student’s t -tests (* P < 0.05, ** P < 0.01, *** P < 0.001).

Techniques: Expressing, Staining, Migration, Immunofluorescence, Two Tailed Test

a – d , Western blotting analysis ( a ) and quantification of CX3CR1 ( b ), p-STAT3 ( c ) and STAT3 expression ( d ) in BV2 cells transfected with siSTAT3 and siCX3CR1 or siCX3CR1 and siCTR. e , qPCR analysis of the expression of the proinflammatory molecules Cx3cr1 and Stat3 in BV2 cells after transfection with siSTAT3 and siCX3CR1 or siCX3CR1 and siCTR. f , qPCR analysis of proinflammatory cytokines in IMA2.1 cells treated with MCM-3, MCM-2 or MCM-1. g , qPCR analysis of A1- and A2-specific gene expression in IMA2.1 cells treated with MCM-3, MCM-2 or MCM-1. h – j , Flow cytometry analysis ( h ) and quantification of the percentages of Annexin-V + /PI − ( i ) and Annexin-V + /PI + ( j ) cells among 661W cells after treatment with MCM-3, MCM-2 or MCM-1. k – l , qPCR analysis of Caspase-3 ( k ) and Bax ( l ) expression in 661W cells treated with MCM-3, MCM-2 or MCM-1. The results shown represent three to five independent experiments. The data are presented as the mean ± s.e.m. and were analyzed via one-way ANOVA with Tukey’s multiple comparison test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Journal: Experimental & Molecular Medicine

Article Title: Microglial CX3CR1 deficiency regulates the selective vulnerability of cone photoreceptors via STAT3/CCL–ACKR1 signaling in the mouse retina

doi: 10.1038/s12276-025-01618-7

Figure Lengend Snippet: a – d , Western blotting analysis ( a ) and quantification of CX3CR1 ( b ), p-STAT3 ( c ) and STAT3 expression ( d ) in BV2 cells transfected with siSTAT3 and siCX3CR1 or siCX3CR1 and siCTR. e , qPCR analysis of the expression of the proinflammatory molecules Cx3cr1 and Stat3 in BV2 cells after transfection with siSTAT3 and siCX3CR1 or siCX3CR1 and siCTR. f , qPCR analysis of proinflammatory cytokines in IMA2.1 cells treated with MCM-3, MCM-2 or MCM-1. g , qPCR analysis of A1- and A2-specific gene expression in IMA2.1 cells treated with MCM-3, MCM-2 or MCM-1. h – j , Flow cytometry analysis ( h ) and quantification of the percentages of Annexin-V + /PI − ( i ) and Annexin-V + /PI + ( j ) cells among 661W cells after treatment with MCM-3, MCM-2 or MCM-1. k – l , qPCR analysis of Caspase-3 ( k ) and Bax ( l ) expression in 661W cells treated with MCM-3, MCM-2 or MCM-1. The results shown represent three to five independent experiments. The data are presented as the mean ± s.e.m. and were analyzed via one-way ANOVA with Tukey’s multiple comparison test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Techniques: Western Blot, Expressing, Transfection, Gene Expression, Flow Cytometry, Comparison

a , Flow cytometry analysis of p-STAT3 expression in microglia (CD11b + ), astrocytes (GFAP + ) and cone photoreceptors (R/G opsin + ) from the retinas of CX3CR1 GFP/GFP and CX3CR1 +/GFP or C57BL/6J mice at 6 weeks of age ( n = 3–4 mice per group). b , Quantification of the percentages of p-STAT3 + /GFP − , p-STAT3 + /GFP + and p-STAT3 − /GFP + cells in a . c , Quantification of the percentages of p-STAT3 + /GFAP − , p-STAT3 + /GFAP + and p-STAT3 − /GFAP + cells in a . d , Quantification of the percentages of p-STAT3 + /R/G-opsin − , p-STAT3 + /R/G opsin + and p-STAT3 − /R/G opsin + cells in a . e , Colocalization of p-STAT3 and GFAP in astrocytes from the retinas of 6-week-old CX3CR1 GFP/GFP or C57BL/6J mice. The white arrowheads indicate p-STAT3 + /GFAP + cells. Scale bar, 50 µm. f , Quantification of the percentages of p-STAT3 + /GFAP + cells in e . g , qPCR analysis of Stat3 and proinflammatory molecule expression in astrocytes from the retinas of 6-week-old CX3CR1 GFP/GFP or C57BL/6J mice. h , i , Western blotting analysis ( h ) and quantification of p-STAT3/STAT3 expression levels ( i ) in IMA2.1 cells treated with Colivelin TFA or PBS. j , qPCR analysis of proinflammatory molecules in IMA2.1 cells after Colivelin TFA or PBS treatment. k , qPCR analysis of A1- and A2-specific gene expression in IMA2.1 cells treated with Colivelin TFA or PBS. l ‒ o , Flow cytometry analysis ( l ) and quantification of the percentages of Annexin-V + /PI − ( m ), Annexin-V + /PI + ( n ) and Annexin-V − /PI + ( o ) cells among 661W cells treated with ACM-2 or ACM-1. The results shown represent three to four independent experiments. The data are presented as the mean ± s.e.m. and were analyzed via unpaired two-tailed Student’s t -tests (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Journal: Experimental & Molecular Medicine

Article Title: Microglial CX3CR1 deficiency regulates the selective vulnerability of cone photoreceptors via STAT3/CCL–ACKR1 signaling in the mouse retina

doi: 10.1038/s12276-025-01618-7

Figure Lengend Snippet: a , Flow cytometry analysis of p-STAT3 expression in microglia (CD11b + ), astrocytes (GFAP + ) and cone photoreceptors (R/G opsin + ) from the retinas of CX3CR1 GFP/GFP and CX3CR1 +/GFP or C57BL/6J mice at 6 weeks of age ( n = 3–4 mice per group). b , Quantification of the percentages of p-STAT3 + /GFP − , p-STAT3 + /GFP + and p-STAT3 − /GFP + cells in a . c , Quantification of the percentages of p-STAT3 + /GFAP − , p-STAT3 + /GFAP + and p-STAT3 − /GFAP + cells in a . d , Quantification of the percentages of p-STAT3 + /R/G-opsin − , p-STAT3 + /R/G opsin + and p-STAT3 − /R/G opsin + cells in a . e , Colocalization of p-STAT3 and GFAP in astrocytes from the retinas of 6-week-old CX3CR1 GFP/GFP or C57BL/6J mice. The white arrowheads indicate p-STAT3 + /GFAP + cells. Scale bar, 50 µm. f , Quantification of the percentages of p-STAT3 + /GFAP + cells in e . g , qPCR analysis of Stat3 and proinflammatory molecule expression in astrocytes from the retinas of 6-week-old CX3CR1 GFP/GFP or C57BL/6J mice. h , i , Western blotting analysis ( h ) and quantification of p-STAT3/STAT3 expression levels ( i ) in IMA2.1 cells treated with Colivelin TFA or PBS. j , qPCR analysis of proinflammatory molecules in IMA2.1 cells after Colivelin TFA or PBS treatment. k , qPCR analysis of A1- and A2-specific gene expression in IMA2.1 cells treated with Colivelin TFA or PBS. l ‒ o , Flow cytometry analysis ( l ) and quantification of the percentages of Annexin-V + /PI − ( m ), Annexin-V + /PI + ( n ) and Annexin-V − /PI + ( o ) cells among 661W cells treated with ACM-2 or ACM-1. The results shown represent three to four independent experiments. The data are presented as the mean ± s.e.m. and were analyzed via unpaired two-tailed Student’s t -tests (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Techniques: Flow Cytometry, Expressing, Western Blot, Gene Expression, Two Tailed Test

a , UMAP plot showing eight unique microglial clusters from CX3CR1 GFP/GFP and C57BL/6J retinas at 6 weeks of age. b , Bubble chart showing marker gene expression in each cluster. c , Volcano plot showing DEGs in cluster aMG_1 ( Tnf dominant). The red dots indicate upregulated DEGs (FC >1.5), and the blue dots indicate downregulated DEGs (FC <1.5). d , PPI network of DEGs in cluster aMG_1 ( Tnf dominant). e , Violin plots showing Cx3cr1 , Tnf , Cd68 and Cxcl1 expression in each cluster. f , KEGG pathway analysis of DEGs in aMG_1 ( Tnf dominant). g , TNF signaling pathway in aMG_1 ( Tnf dominant) by GSEA. h , UMAP plots showing the AUC activities of TNF signaling in microglia from CX3CR1 GFP/GFP and C57BL/6J retinas. i, Colocalization of Iba-1 with CD68, TNF-α or CXCL1 in MACS-sorted microglia from CX3CR1 GFP/GFP and C57BL/6J retinas at 6 weeks of age. Scale bar, 20 µm. j , Activity of the top 20 TFs in 1,000 microglia from CX3CR1 GFP/GFP and C57BL/6J retinas at 6 weeks of age. k , Distribution of 8 different clusters among 1,000 microglia from CX3CR1 GFP/GFP and C57BL/6J retinas. l , Extended regulon activity of Stat3 among 1,000 microglia. m , Stat3 and Kdm6b expression in each cluster. n – r , Flow cytometry analysis ( n ) and quantification of CD11b-FITC + ( o ) and p-STAT3-APC + ( p ), TNF-α-Alexa Fluor-594 + ( q ) and p-STAT3-APC +/ TNF-α-Alexa Fluor-594 + ( r ) cells among CD11b + microglia from CX3CR1 GFP/GFP and C57BL/6J retinas at 6 weeks of age ( n = 4 mice per group). The data are presented as the mean ± s.e.m. and were analyzed via unpaired two-tailed Student’s t -tests (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). s , LR interaction network among eight different clusters. t , Circle plot showing TNF signaling networks. u , Heatmap showing the relative importance of each cluster on the basis of the computed four network centrality measures of TNF signaling. v , Pseudotime trajectory analysis of microglia.

Journal: Experimental & Molecular Medicine

Article Title: Microglial CX3CR1 deficiency regulates the selective vulnerability of cone photoreceptors via STAT3/CCL–ACKR1 signaling in the mouse retina

doi: 10.1038/s12276-025-01618-7

Figure Lengend Snippet: a , UMAP plot showing eight unique microglial clusters from CX3CR1 GFP/GFP and C57BL/6J retinas at 6 weeks of age. b , Bubble chart showing marker gene expression in each cluster. c , Volcano plot showing DEGs in cluster aMG_1 ( Tnf dominant). The red dots indicate upregulated DEGs (FC >1.5), and the blue dots indicate downregulated DEGs (FC <1.5). d , PPI network of DEGs in cluster aMG_1 ( Tnf dominant). e , Violin plots showing Cx3cr1 , Tnf , Cd68 and Cxcl1 expression in each cluster. f , KEGG pathway analysis of DEGs in aMG_1 ( Tnf dominant). g , TNF signaling pathway in aMG_1 ( Tnf dominant) by GSEA. h , UMAP plots showing the AUC activities of TNF signaling in microglia from CX3CR1 GFP/GFP and C57BL/6J retinas. i, Colocalization of Iba-1 with CD68, TNF-α or CXCL1 in MACS-sorted microglia from CX3CR1 GFP/GFP and C57BL/6J retinas at 6 weeks of age. Scale bar, 20 µm. j , Activity of the top 20 TFs in 1,000 microglia from CX3CR1 GFP/GFP and C57BL/6J retinas at 6 weeks of age. k , Distribution of 8 different clusters among 1,000 microglia from CX3CR1 GFP/GFP and C57BL/6J retinas. l , Extended regulon activity of Stat3 among 1,000 microglia. m , Stat3 and Kdm6b expression in each cluster. n – r , Flow cytometry analysis ( n ) and quantification of CD11b-FITC + ( o ) and p-STAT3-APC + ( p ), TNF-α-Alexa Fluor-594 + ( q ) and p-STAT3-APC +/ TNF-α-Alexa Fluor-594 + ( r ) cells among CD11b + microglia from CX3CR1 GFP/GFP and C57BL/6J retinas at 6 weeks of age ( n = 4 mice per group). The data are presented as the mean ± s.e.m. and were analyzed via unpaired two-tailed Student’s t -tests (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). s , LR interaction network among eight different clusters. t , Circle plot showing TNF signaling networks. u , Heatmap showing the relative importance of each cluster on the basis of the computed four network centrality measures of TNF signaling. v , Pseudotime trajectory analysis of microglia.

Techniques: Marker, Gene Expression, Expressing, Activity Assay, Flow Cytometry, Two Tailed Test

a , UMAP plot showing different retinal cell clusters from the retinas of 6-week-old CX3CR1 GFP/GFP and C57BL/6J mice. b , Marker gene expression in each cluster. c , Pseudotime analysis of six distinct cone cell clusters. d , Expression of phototransduction-associated genes ( Gnat , Gnb1 , Nr2e3 , Nrl and Pdc ) and apoptosis-associated genes ( Egr1 , Fos , Gabrb3 , Gsdme and Prnp ) in cones over pseudotime. e , Inflammation-related gene expression in astrocytes from the retinas of 6-week-old CX3CR1 GFP/GFP and C57BL/6J mice. f , Cell‒cell interactions between microglia and other retinal cells. g , Signaling changes in cones from the retinas of 6-week-old CX3CR1 GFP/GFP and C57BL/6J mice. h , LR interactions between microglia and cones. i , Violin plots showing CCL and CXCL signaling expression in different retinal cells. j , Expression of NF-ĸB signaling-associated DEGs in cones, bipolar cells, amines and RGCs between CX3CR1 GFP/GFP and C57BL/6J retinas. k , l , Flow cytometry analysis ( k ) and quantification ( l ) of the percentages of Annexin-V + /PI − , Annexin-V + /PI + and Annexin-V − /PI + cells among 661W cells pretreated with siAckr1 or siCTR and cocultured with pMCM-2 or pMCM-1. m , qPCR analysis of Ackr1 , Caspase-3 and Bax expression in 661W cells treated as described in k . n , o , Flow cytometry analysis ( n ) and quantification ( o ) of the percentages of Annexin-V + /PI − , Annexin-V + /PI + and Annexin-V − /PI + cells among 661W cells treated with CCL2 (100 ng/ml) after pretreatment with siAckr1 or siCTR. p , qPCR analysis of Ackr1 , Caspase-3 and Bax in 661W cells treated as described in n . The results represent three independent experiments. The data are presented as the mean ± s.e.m. and were analyzed via one-way ANOVA with Tukey’s multiple comparison test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Journal: Experimental & Molecular Medicine

Article Title: Microglial CX3CR1 deficiency regulates the selective vulnerability of cone photoreceptors via STAT3/CCL–ACKR1 signaling in the mouse retina

doi: 10.1038/s12276-025-01618-7

Figure Lengend Snippet: a , UMAP plot showing different retinal cell clusters from the retinas of 6-week-old CX3CR1 GFP/GFP and C57BL/6J mice. b , Marker gene expression in each cluster. c , Pseudotime analysis of six distinct cone cell clusters. d , Expression of phototransduction-associated genes ( Gnat , Gnb1 , Nr2e3 , Nrl and Pdc ) and apoptosis-associated genes ( Egr1 , Fos , Gabrb3 , Gsdme and Prnp ) in cones over pseudotime. e , Inflammation-related gene expression in astrocytes from the retinas of 6-week-old CX3CR1 GFP/GFP and C57BL/6J mice. f , Cell‒cell interactions between microglia and other retinal cells. g , Signaling changes in cones from the retinas of 6-week-old CX3CR1 GFP/GFP and C57BL/6J mice. h , LR interactions between microglia and cones. i , Violin plots showing CCL and CXCL signaling expression in different retinal cells. j , Expression of NF-ĸB signaling-associated DEGs in cones, bipolar cells, amines and RGCs between CX3CR1 GFP/GFP and C57BL/6J retinas. k , l , Flow cytometry analysis ( k ) and quantification ( l ) of the percentages of Annexin-V + /PI − , Annexin-V + /PI + and Annexin-V − /PI + cells among 661W cells pretreated with siAckr1 or siCTR and cocultured with pMCM-2 or pMCM-1. m , qPCR analysis of Ackr1 , Caspase-3 and Bax expression in 661W cells treated as described in k . n , o , Flow cytometry analysis ( n ) and quantification ( o ) of the percentages of Annexin-V + /PI − , Annexin-V + /PI + and Annexin-V − /PI + cells among 661W cells treated with CCL2 (100 ng/ml) after pretreatment with siAckr1 or siCTR. p , qPCR analysis of Ackr1 , Caspase-3 and Bax in 661W cells treated as described in n . The results represent three independent experiments. The data are presented as the mean ± s.e.m. and were analyzed via one-way ANOVA with Tukey’s multiple comparison test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Techniques: Marker, Gene Expression, Expressing, Flow Cytometry, Comparison

a , LR interactions between microglia and astrocytes. b , Upregulated signaling between microglia and astrocytes. c , Bmp signaling expression in different retinal cell clusters. d , Bmp2 expression in MACS-sorted microglia from CX3CR1 GFP/GFP and C57BL/6J mouse retinas. e , Bmp2 expression in BV2 cells following treatment with the recombinant CXCL1 protein (100 ng/ml) . f , qPCR analysis of Bmpr1a , Bmpr1b , Tnfα , Il1b and Il6 expression in IMA2.1 cells cocultured with cxMCM-2 or cxMCM-1 after pretreatment with siBmpr1a, siBmpr1b or siCTR. g , h , Western blotting analysis ( g ) and quantification ( h ) of p-STAT3 and STAT3 expression in IMA2.1 cells cocultured with cxMCM-2 or cxMCM-1 after pretreatment with siBmpr1a, siBmpr1b or siCTR. i , j , ELISA analysis of CXCL12 ( i ) and CCL2 ( j ) expression in cell supernatants from IMA2.1 cells cocultured with cxMCM-2 or cxMCM-1 after pretreatment with siBmpr1a, siBmpr1b or siCTR. The results shown represent three to four independent experiments. The data are presented as the mean ± s.e.m. and were analyzed via one-way ANOVA with Tukey’s multiple comparison test or unpaired two-tailed Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). k , Schematic illustration showing the role of CX3CR1/STAT3/CCL–ACKR1 signaling in mediating the selective vulnerability of cone photoreceptors in the mouse retina.

Journal: Experimental & Molecular Medicine

Article Title: Microglial CX3CR1 deficiency regulates the selective vulnerability of cone photoreceptors via STAT3/CCL–ACKR1 signaling in the mouse retina

doi: 10.1038/s12276-025-01618-7

Figure Lengend Snippet: a , LR interactions between microglia and astrocytes. b , Upregulated signaling between microglia and astrocytes. c , Bmp signaling expression in different retinal cell clusters. d , Bmp2 expression in MACS-sorted microglia from CX3CR1 GFP/GFP and C57BL/6J mouse retinas. e , Bmp2 expression in BV2 cells following treatment with the recombinant CXCL1 protein (100 ng/ml) . f , qPCR analysis of Bmpr1a , Bmpr1b , Tnfα , Il1b and Il6 expression in IMA2.1 cells cocultured with cxMCM-2 or cxMCM-1 after pretreatment with siBmpr1a, siBmpr1b or siCTR. g , h , Western blotting analysis ( g ) and quantification ( h ) of p-STAT3 and STAT3 expression in IMA2.1 cells cocultured with cxMCM-2 or cxMCM-1 after pretreatment with siBmpr1a, siBmpr1b or siCTR. i , j , ELISA analysis of CXCL12 ( i ) and CCL2 ( j ) expression in cell supernatants from IMA2.1 cells cocultured with cxMCM-2 or cxMCM-1 after pretreatment with siBmpr1a, siBmpr1b or siCTR. The results shown represent three to four independent experiments. The data are presented as the mean ± s.e.m. and were analyzed via one-way ANOVA with Tukey’s multiple comparison test or unpaired two-tailed Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). k , Schematic illustration showing the role of CX3CR1/STAT3/CCL–ACKR1 signaling in mediating the selective vulnerability of cone photoreceptors in the mouse retina.

Techniques: Expressing, Recombinant, Western Blot, Enzyme-linked Immunosorbent Assay, Comparison, Two Tailed Test

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